Am J Med. Jun 15;(12) Cost-effectiveness of testing for hypercoagulability and effects on treatment strategies in patients with deep vein. As for affinity maturation, which is the process to increase their affinity for a particular antigen via their B cells during the course of an immune response.
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With years of experience, we can offer high-quality phage display ce construction and custom phage display library screening services to meet your various demands precisely.
We are confident that our phage display technologies could allow you have new opportunities in immunotherapy, drug discovery, and functional genomics.
Phage display is the technology that inserts the DNA coufs of foreign protein or peptide into the appropriate position of phage coat protein structural gene, so that the exogenous gene can be expressed along with the expression of phage coat protein itself.
Meanwhile, the foreign protein also can be shown in the surface of phage coat protein following the reassembly of phage.
In short, a phage library expressing a wide diversity of peptides or proteins is used to select those binding the desired targets. Based on different phages, vectors or libraries, the phage display systems could be divided into various types, details as below. CUSABIO could provide phage display technologies to customers that cover the broad scope of the technologies including key areas of library construction and screening as well as antibody modification and expression.
The phage display library technology refers to the clone and expression for variable region genes of all antibodies from a certain animal in a plasmid or phage, then cougs clones carrying specific antibody genes via different antigens, thereby, to obtain corresponding specific antibodies or peptides. CUSABIO is committed re offering quality services of antibody library construction as well as peptide library construction to customers.
CUSABIO is mainly engaged in offering quality and personalized services of antibody library screening as well as peptide library screening to meet skr various demands.
CUSABIO can construct antibody libraries as well as screen desired monoclonal antibodies with high specificity and affinity from a wide range of species, such as human, alpaca, mouse, rabbit, chicken, dog, bovine, and so on.
Compared with the traditional hybridoma method, antibody phage display library has distinct advantages on selecting novel monoclonal antibodies and even the fully humanized antibody. Furthermore, the antibodies from phage library have following advantages.
As we known, peptides are biological molecules, consisting of short sequences of amino acids, so peptides are easy to be synthesized, show low toxicity and high efficiency. Most importantly, if targeting receptors or molecules that are specially expressed on cancer cells would significantly enhance cancer therapeutic effect and reduce cancer-related mortality as well. Thus phage display technology on screening target peptides for developing cancer drugs absolutely can offer new opportunities for the treatment of many tumor diseases.
Humanized antibodies are antibodies from non-human species whose protein sequences have been modified to increase their similarity to antibody variants produced naturally in human beings. As for affinity maturation, which is the process to increase their affinity for a particular antigen via their B cells during the course of an immune response.
With repeated processes of somatic hypermutation for B-cell receptors and subsequent clonal selection to the same antigen, a host will produce antibodies of successively greater affinities.
Antibody humanization has played a fundamental role in the remarkable progress of antibodies as therapeutic reagents.
CUSABIO Phage Display Service
As we known, since the advent of hybridoma technology, most monoclonal antibodies derive from rodent, such as mouse, rat, rabbit, hamster, etc. But most rodent antibodies have been shown a limited use as therapeutic agents because of a short serum half-life as well as they can not trigger human effector functions. On the other hand, in some cases, the affinity of a certain antibody deriving from phage library may not be high enough for used as therapeutic agents, then affinity maturation is urgently required to preserve the epitope specificity and functional activity of the antibody as well as boost potency to the desired level.
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Your Good Partner in Biology Research. Protocols References Download Center. Technical Articles A collection of articles that focus on an array of different scientific topics such as pathways, cancer, transmembrane proteins. Contact Us Distributors Worldwide. Introduction of Phage Display Technology Phage display is the technology that inserts the DNA sequence of foreign protein or peptide into the appropriate position of phage coat protein structural gene, so that the exogenous gene can be expressed along with the expression of phage coat protein itself.
Classification of Phage Display Systems Based on different phages, vectors or libraries, the phage display systems could be divided into various types, details as below.
Phage Display Library Construction The phage display library technology refers to the clone and expression for variable region genes of all antibodies from a certain animal in a plasmid or phage, then screen clones carrying specific antibody genes via different antigens, thereby, to obtain corresponding specific antibodies or peptides. Process Frendt Items Service Cycle 1 Separation of lymphocytes Obtain lymphocytes from peripheral blood, spleen or bone marrow.
5. mécanique de Newton – exovideo Terminale S
Phage Display Library Screening CUSABIO is mainly engaged in offering quality and personalized services of antibody library screening as well as peptide library screening to meet customers’ various demands. Direct screening of human antibodies. Screening antibodies that recognize toxicity or autoantigens. Obtaining antibody genes for further direct modification.
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With cDNA as the template, the antibody gene will be extended with degenerate primer, then inserted into the phage vector. Detect antibody library capacity via colony counting, as well as rrepere antibody library diversity via sequencing. Antibody sequencing report; Antibody sequence analysis report; antibodies with best affinity.